Spectroscopy Ii- Hplc & Raman

UNIVERSITI TUNKU ABDUL RAHMAN Faculty Course Year/ Semester Session : : : : Faculty of erudition Bac. of Science (Hons) Chemistry Year 3 Semester 1 201105 or May 2011 Unit Code Unit Title subscriber Assignment : : : : UDEC3254 Spectroscopy and Chromatography II Dr. Lim Chan Kiang 2 coiffure completely questions 1. A diverseness of steroid hormones consisting of cortisone, cortexone and corticosterone was separated utilize a HPLC silica column eluted with a binary mixture of dimethyl ketone and hexane (20:80). (i) What is the ordination of elution sequence for the above compounds from the HPLC column? (3 marks) (ii) What is the chromatographic chemical mechanism involved? get out how the mechanism make separation to the threesome compounds. (8 marks) (iii) With explanation, describe what would happen to the peaks in the chromatogram when the wadding silica with a larger pore size and little surface stadium is used in the above separation. (6 marks) 2. A mixture of Br-, Cl- and PO43- ions was subjected to an non-ionic detergent column packed with polym-N(CH3)3+OH-, eluted with 9mM sodium carbonate. (i) raise the above analyte ions in order of increasing retention succession in the anionic detergent column.

(3 marks) (ii) Describe how the analyte ions were separated in the anionic column harmonise to order of retention time answered in part (2)(i). (8 marks) 3. An stranger compound is irradiated with an excitation photon. An anti-Stokes chicken out out is observed at 439.3 nm corresponding to sacque of 2270 cm-1. i) What is the excitation photon used in the barb above? (2 marks) ii) date the wavenumber and wavelength of the St! okes line and anti-Stokes line with shifts of 3270 cm-1 and 1170 cm-1, respectively. (6 marks) iii) A Stokes line supposed to be found in the region 490 nm to 500 nm in the Raman spectrum was swamped by fluorescent band. Describe how to solve the conundrum so that the Raman shift for the Stokes line usher out be determined. (4 marks) If you want to get a full essay, order it on our website: BestEssayCheap.com

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